Review



mouse anti htfr pe conjugate  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    R&D Systems mouse anti htfr pe conjugate
    Mouse Anti Htfr Pe Conjugate, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+htfr+pe+conjugate/Human+TfR+(Transferrin+R)+PE-conjugated+Antibody/us10233252-441-20-25
    Average 91 stars, based on 7 article reviews
    mouse anti htfr pe conjugate - by Bioz Stars, 2026-10
    91/100 stars

    Images

    Related Articles

    Purification:

    Article Title: Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources
    Article Snippet: .. Two hundred microliters of purified scFv at a concentration of 200 nM (an amount known to saturate available 9E10 on the bead surface) were added to each experimental well and allowed to equilibrate for 2 h. The particles were washed once with TBSA, resuspended in 200 μl of rhTfR (40 nM, saturating) and equilibrated for 1 h. Particle-bound scFv was detected using a rabbit anti-his6 polyclonal antibody (Thermo-Fisher, diluted 1:100) followed by goat anti-rabbit APC conjugate (Invitrogen, diluted 1:500). rhTfR binding was detected by mouse anti-hTfR PE conjugate (R&D systems, diluted 1:10). ..

    Concentration Assay:

    Article Title: Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources
    Article Snippet: .. Two hundred microliters of purified scFv at a concentration of 200 nM (an amount known to saturate available 9E10 on the bead surface) were added to each experimental well and allowed to equilibrate for 2 h. The particles were washed once with TBSA, resuspended in 200 μl of rhTfR (40 nM, saturating) and equilibrated for 1 h. Particle-bound scFv was detected using a rabbit anti-his6 polyclonal antibody (Thermo-Fisher, diluted 1:100) followed by goat anti-rabbit APC conjugate (Invitrogen, diluted 1:500). rhTfR binding was detected by mouse anti-hTfR PE conjugate (R&D systems, diluted 1:10). ..

    Binding Assay:

    Article Title: Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources
    Article Snippet: .. Two hundred microliters of purified scFv at a concentration of 200 nM (an amount known to saturate available 9E10 on the bead surface) were added to each experimental well and allowed to equilibrate for 2 h. The particles were washed once with TBSA, resuspended in 200 μl of rhTfR (40 nM, saturating) and equilibrated for 1 h. Particle-bound scFv was detected using a rabbit anti-his6 polyclonal antibody (Thermo-Fisher, diluted 1:100) followed by goat anti-rabbit APC conjugate (Invitrogen, diluted 1:500). rhTfR binding was detected by mouse anti-hTfR PE conjugate (R&D systems, diluted 1:10). ..

    Article Title: Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation
    Article Snippet: .. Particle-bound scFv was detected using a DyLight650-conjugated anti-his6 antibody (Abcam, ab117504, diluted 1:300 in TBSA). rhTfR binding was detected by mouse anti-hTfR PE conjugate (FAB2474P, R&D systems, diluted 1:10 in TBSA). .. MFIs of the labeled beads were quantified using FlowJo; the bound rhTfR per scFv on the bead was determined as the ratio of PE fluorescence (TfR binding) divided by the DyLight650 fluorescence (scFv amount).

    Article Title: Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation.
    Article Snippet: .. Particle-bound scFv was detected using a DyLight650-conjugated anti-his6 antibody (Abcam, ab117504, diluted 1:300 in TBSA). rhTfR binding was detected by mouse anti-hTfR PE conjugate (FAB2474P, R&D systems, diluted 1:10 in TBSA). .. MFIs of the labeled beads were quantified using FlowJo; the bound rhTfR per scFv on the bead was determined as the ratio of PE fluorescence (TfR binding) divided by the DyLight650 fluorescence (scFv amount).

    Article Title: pH-dependent antibodies targeting the transferrin receptor and methods of use thereof to deliver a therapeutic agent
    Article Snippet: .. Particle-bound scFv was detected using a DyLight650-conjugated anti-his6 antibody (Abcam, ab117504, diluted 1:300 in TBSA). rhTfR binding was detected by mouse anti-hTfR PE conjugate (FAB2474P, R&D systems, diluted 1:10 in TBSA). .. MFIs of the labeled beads were quantified using FlowJo; the bound rhTfR per scFv on the bead was determined as the ratio of PE fluorescence (TfR binding) divided by the DyLight650 fluorescence (scFv amount).



    Similar Products

    91
    R&D Systems mouse anti htfr pe conjugate
    Mouse Anti Htfr Pe Conjugate, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+htfr+pe+conjugate/Human+TfR+(Transferrin+R)+PE-conjugated+Antibody/us10233252-441-20-25
    Average 91 stars, based on 1 article reviews
    mouse anti htfr pe conjugate - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    Image Search Results